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| Registros recuperados: 269 | |
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| Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney. |
| Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative... |
| Tipo: Text |
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf |
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| Gasparotto,Elainy Patricia Lino; Tognon,Raquel; Ferreira,Aline Fernanda; Oliveira,Gislane Lelis Vilela; Palma,Patrícia Vianna Bonini; Zanichelli,Maria Aparecida; Souto,Elizabeth Xisto; Velano,Carlos Eduardo Engel; Simões,Belinda Pinto; Carrara,Rita de Cassia Viu; Kashima,Simone; Covas,Dimas Tadeu; Castro,Fabíola Attie de; Souza,Ana Maria de. |
| Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polycythemia vera; Gene mutation; Gene expression; Apoptosis; Bcl-2 family members. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400025 |
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| Simão,T.A.; Andrada-Serpa,M.J.; Mendonça,G.A.S.; Marques,D.D.; Braga,M.A.; Santos,A.L.S.; Amorim,L.M.F.; Cardoso,L.E.B.; Carmo,P.A.O.; De-Moura-Gallo,C.V.. |
| Apoptosis is a well-known specific process of cell death that normally occurs in physiological situations such as tissue or organ development and involution. During tumor growth there is a balance between proliferation and cell death which involves apoptotic mechanisms. In the present study genomic DNAs from 120 breast tumor biopsies were analyzed by agarose gel electrophoresis and none of them presented the fragmentation pattern characteristic of the apoptosis process. However, 33% of the 105 breast cancer patients clearly showed the apoptotic pattern when DNA from blood cells was analyzed. None of the DNAs from healthy volunteer blood cells showed any trace of apoptosis. Since the breast cancer patients were not receiving chemo- or hormone therapy, the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Apoptosis; Breast cancer; Peripheral blood cells; Cortisol; Fas-system. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400004 |
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| Moro,L.; Vasconcelos,A.C.; Santos,F.G.A.; Alves,C.M.; Nunes,J.E.S.; Sampaio,I.B.M.. |
| Determinou-se o número mínimo de campos por lâmina para se ter uma amostragem representativa para o estudo de apoptose em linfonodos de cães com cinomose. Um linfonodo traqueobrônquico de cão foi colhido e processado segundo técnica para inclusão em parafina. Secções de 5µm foram coradas pelo tricrômico de Shorr. Utilizou-se um analisador de imagens para registrar o número de células em apoptose e os índices apoptóticos de 300 campos, na mesma lâmina. Obtiveram-se, então, valores médios para 10 amostras de 5, 10, 15, 20, 25, 30, 35, 40, 45 e 50 campos ao acaso. Realizou-se o estudo dos desvios-padrão das médias (DP) em relação ao tamanho da amostra que os originou. Quando as oscilações de DP entre amostras consecutivas foram menores que 5%, o ganho em... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dog; Apoptosis; Morphometry; Lymph node; Sampling. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300020 |
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| SOARES,JULIANA M.; FARIA,BRUNA M. DE; ASCARI,LUCAS M.; ALVES-LEON,SONIZA V.; SOUZA,JORGE M. DE; SOARES,ANTONIO G.; CORDEIRO,YRAIMA; ROMÃO,LUCIANA F.. |
| Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Diosmin; Flavonoid; Glioblastoma. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700701 |
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| Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L.. |
| Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548 |
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| Yingchun,Li; Xiujuan,Qu; Jinglei,Qu; Ye,Zhang; Jing,Liu; Yuee,Teng; Xuejun,Hu; Kezuo,Hou; Yunpeng,Liu. |
| Arsenic trioxide (ATO) is a strong inducer of apoptosis in malignant hematological cells. Inducible phosphatidyl inositol 3 kinase (PI3K)-Akt activation promotes resistance to ATO. In the present study, we evaluated whether E3 ubiquitin ligase Cbl-b, a negative regulator of PI3K activation, is involved in the action of ATO. The effect of ATO on cell viability was measured by the Trypan blue exclusion assay or by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was determined by flow cytometry and protein expression was assayed by Western blotting. ATO decreased the viability of HL60 cells and induced cellular apoptosis, which was accompanied by transient activation of Akt. The PI3K/Akt inhibitor, LY294002,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arsenic trioxide; Apoptosis; Cbl-b; PI3K/Akt pathway. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200004 |
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| García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F.. |
| Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002 |
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| Zong,L.; Yu,Q.H.; Du,Y.X.; Deng,X.M.. |
| Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Edaravone; Apoptosis; Endotoxin; Liver injury; Inflammation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300231 |
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| Jiménez Venegas,Leonella; Quilodrán Peredo,Javier; Miranda Olivares,Juan Pablo; Rodríguez Bustos,Héctor. |
| La cipermetrina es un pesticida ampliamente utilizado en agricultura y en salud pública. Sus efectos histopatológicos a nivel de sistema nervioso central han sido poco estudiados, aún cuando se han demostrado casos de intoxicación en humanos, con importante compromiso neurológico. El propósito de este trabajo es conocer los efectos de una dosis única intraperitoneal de cipermetrina sobre la morfología neuronal de corteza somatosensorial (láminas superficiales y profundas) de ratones CF-1. 35 ratones fueron separados en tres grupos: control (n=5), control vehículo (n=15) y experimental (n=15); los dos últimos grupos sacrificados los días 1, 8, 18, 26 y 35, en grupos de a tres. Los encéfalos fueron removidos, fijados, y procesados con técnica de rutina, para... |
| Tipo: Journal article |
Palavras-chave: Corteza somatosensorial; Apoptosis; Cipermetrina; Neurotoxicidad. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100003 |
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| Shu,J.C.; He,Y.J.; Lv,X.; Zhao,J.R.; Zhao,J.; Shen,Y.; Ye,G.R.; Wang,L.X.. |
| This study was designed to investigate the effect of curcumin (diferuloylmethane) on the proliferation and apoptosis of hepatic stellate cells (HSC). The cell line HSC-T6 (1.25 x 10(5) cells/mL) was incubated with curcumin and HSC proliferation was detected by a methyl thiazolyl tetrazolium colorimetric assay. HSC apoptosis was detected by flow cytometry, transmission electron microscope and agarose gel electrophoresis. HSC proliferation was significantly inhibited in a concentration-dependent manner (10.6 to 63.5%) after incubation with 20-100 μM curcumin, compared with a control group. At 20, 40, and 60 μM, after 24 h of incubation, curcumin was associated with a significant increase in the number of HSC in the G2/M phase, and a significant decrease in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcumin; Hepatic stellate cells; Proliferation; Apoptosis; Hepatic fibrosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200009 |
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| Jin,Xiao-Dong; Cao,Min; Zhou,Xie-Lai; Yin,Hong-Ping; Chen,Zhao-Dian; Xu,Ning; Jiang,Hai. |
| We investigated the contribution of the duration of overdistention (DOD) to rat bladder function and morphology and explored its possible molecular mechanisms. Bladder overdistention was induced in male Sprague-Dawley rats (200-250 g) by an infusion of saline. Forty rats were divided into 5 groups submitted to different DOD, i.e., 1, 2, 4, and 8 h, and control. Bladder function was evaluated by cystometry. Morphological changes were observed by light and transmission electron microscopy. Compared to control (44.567 ± 3.472 cmH2O), the maximum detrusor pressure of groups with 2-, 4- and 8-h DOD decreased significantly (means ± SEM): 32.774 ± 3.726, 31.321 ± 2.847, and 29.238 ± 3.724 cmH2O. With the increase of DOD, inflammatory infiltration and impairment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute urinary retention; Bladder overdistention; Urodynamics; Ultrastructure; Apoptosis; Rat. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000006 |
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| Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫. |
| Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3... |
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Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922 |
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| Treitinger,Aricio; Spada,Celso; Masokawa,Ivete Yoshico; Verdi,Júlio César Vidal; Silveira,Mariette Van Der Sander; Luis,Magali Chaves; Reis,Marcellus; Ferreira,Silvia Inês Alejandra Cordova de Pires; Abdalla,Dulcinéia Saes Parra. |
| N-acetyl-L-cysteine (NAC) has been proposed as an additional therapeutic agent for AIDS patients because it reduces human immunodeficiency virus type 1 (HIV-1) replication in stimulated CD4+ lymphocytes, and it ameliorates immunological reactivity. In a randomized, 180-day, double-blind, placebo-controlled trial performed with HIV-infected patients classified as A2 and A3 according to the criteria of the Center for Disease Control and Prevention, we investigated the effects of oral administration of NAC on HIV-infected patients undergoing their first anti-retroviral therapy; viral load, CD4+ lymphocyte, lymphocyte viability and apoptosis, and TNF-alpha and IL-8 levels were determined. Sixteen patients who received anti-retroviral therapy plus a placebo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: N-acetylcysteine; Apoptosis; Viability; HIV; Glutathione; TNF-alpha; IL-8. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500005 |
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| Registros recuperados: 269 | |
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